Studies in detoxication. 35. Spectrophotometric determination of β-glucuronidase using p-chlorophenylglucuronide as substrate
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منابع مشابه
Continuous Spectrophotometric Assay for β - Glucuronidase
A continuous spectrophotometric assay has been developed for detecting β-glucuronidase activity. In the assay, Para-nitrophenyl β-D-glucuronide is cleaved to yield a chromophoric product. With the commercial E. coli enzyme, it is demonstrated that the reactions can be continuously monitored by the increase of absorbance at 405 nm. The method is highly sensitive and able to detect less than 1.4 ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1951
ISSN: 0306-3283
DOI: 10.1042/bj0480537